Transmission of phage by glassy-winged sharpshooters, a vector of Xylella fastidiosa
نویسندگان
چکیده
Xylella fastidiosa subsp. fastidiosa (Xff) is the causal agent of Pierce's Disease (PD) of grapevines and is vectored by the glassy-winged sharpshooter (GWSS, Homalodisca vitripennis). Previously we have reported the development of a bacteriophage (phage) based biocontrol system for PD, but no information on insect transmission of phages has been reported. Here we communicate that laboratory reared GWSSs fed on cowpea plants (Vigna unguiculata subsp. unguiculata) harboring the virulent phage Paz were able to uptake of phage efficiently when the phage was present in high concentration, but were inefficient in transfer to plants.
منابع مشابه
INSECT-SYMBIOTIC BACTERIA INHIBITORY TO XYLELLA FASTIDIOSA (PARATRANSGENESIS FOR CONTROL OF PIERCE’S DISEASE): IDENTIFICATION OF ENDOPHYTIC BACTERIA CYCLED BY GLASSY-WINGED SHARPSHOOTERS TO HOST PLANTS Project Leader:
INTRODUCTION Homalodisca coagulata Say, the glassy-winged sharpshooter (GWSS) is known to transmit the etiological agent of Pierce’s disease, Xylella fastidiosa. A paratransgenic approach designed to disrupt the ability of the insect vector to transmit the pathogen involves finding bacterial candidates that possess some degree of intimacy either within the insect and/or host plant xylem. Once a...
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Pymetrozine is a compound that interferes with insect feeding and interrupts transmission of plant pathogens. The glassy-winged sharpshooter, Homalodisca coagulata Say (Hemiptera, Cicadellidae), is a vector of Xylella fastidiosa, the foregut-borne, propagative bacterium that causes Pierce's disease of grapevine. In this study, we recorded the behavioral response of H. coagulata to plants treate...
متن کاملInterim Progress Report for CDFA Agreement Number 12-0116-SA Title of Project Blocking Xylella fastidiosa transmission Principal Investigator
The goal of this project is to develop a technology to block the vector transmission of Xylella fastidiosa to plants. The approach focuses on the disruption of X. fastidiosa-vector interactions, so that the transmission of X. fastidiosa from one plant to another is affected. Our work has demonstrated that this approach is feasible and that we can disrupt sharpshooter transmission of X. fastidio...
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In this project, studies were conducted to 1) develop a method to detect and differentiate multiple strains of Xylella fastidiosa in individual glassy-winged sharpshooters (GWSS,) and 2) determine the relative ability of an individual GWSS to simultaneously retain or transmit two strains of X. fastidiosa. Strain-specific primers were developed that can detect and differentiate the Pierce’s dise...
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